第一部分  显微技术和微生物形态学

PART ONE Microscopic Techniques and Microbial Morphology

  实验一  明视野光学显微镜

  EXPERIMENT 1 Bright--Field Light Microscope

  实验二  暗视野光学显微镜

  EXPERIMENT 2 Dark--Field Light Microscope

  实验三  单染色法

  EXPERIMENT 3 Simple Stain

  实验四  革兰染色法

  EXPERIMENT 4  Gram's Stain

  实验五  芽孢染色法

  EXPERIMENT 5 Spore Stain

  实验六  荚膜染色法

  EXPERIMENT 6 Capsule Stain

  实验七  鞭毛染色法

  EXPERIMENT 7 Flagella Stain

  实验八  螺旋体的染色和形态观察

  EXPERIMENT 8 Spirochetes Morphology

  实验九  悬滴法观察细菌的动力

  EXPERIMENT 9  Observation of Bacterial Motion with Hanging Drop

  实验十  酵母菌的形态观察

  EXPERIMENT 10  Yeast Morphology

  实验十一  微生物大小的测定

  EXPERIMENT 11  Determination of the Size of Microorganisms

  实验十二  放线菌、霉菌的菌丝和孢子形态观察

  EXPERIMENT 12  Morphology of Mycelia and Spore for Actinomycetes and Molds

  实验十三  细菌、放线菌、酵母菌和霉菌的菌落特征观察

  EXPERIMENT 13  Colony Characteristics of Bacteria，Actinomycetes，

    Yeasts and Molds

第二部分  基本实验操作与培养技术

PART TWO Basic Laboratory and Culture Techniques

  实验十四  培养基的配制与灭菌

  EXPERIMENT 14  Culture Media Preparation and Sterilization

  实验十五接种技术与微生物的分离纯化

  EXPERIMENT 15  Culture Transfer Techniques and Isolation of Pure Cultures

  实验十六  常用的消毒灭菌方法

  EXPERIMENT 16  Commonly Used Sterilization and Disinfection Methods

  实验十七  菌种保藏

  EXPERIMENT 17  Preservation of Pure Cuhures

  实验十八  微生物计数法

  EXPERIMENT 18  Counting of Microorganisms

  实验十九  细菌的生长曲线测定

  EXPERIMENT 19  Bacteria Growth Curve

第三部分  细菌的生理生化反应

  PART THREE  Biochemical Activities of Bacteria

  实验二十  淀粉水解和明胶液化试验

  EXERIMENT 20  Hydrolysis of Starch and Protein

  实验二十一  糖发酵试验

  EXERIMENT 21  Utilization of Carbohydrates

  实验二十二  IMViC试验

  EXERIMENT 22  IMViC Series

  实验二十三  硫化氢产生试验和触酶试验

  EXERIMENT 23  Hydrogen sulfide Production and Catalase Test

第四部分  病毒学实验

PART FOUR  Experiments for Virology

  实验二十四  流感病毒的培养与检测

  Experiment 24  Culture and Detection of Influenza Virus

  实验二十五  噬菌体分离和效价测定

  Experiment 25  Isolation and Titer Detection of Bacteriophage

  实验二十六  噬菌体一步生长曲线的测定

  Experiment 26  One——Step Growth Curve of Bacteriophage

第五部分  微生物遗传学实验

PART FIVE  Experiments for Microbial Genetics

  实验二十七  微生物的遗传与变异现象

  EXPERIMENT 27  Heredity and Variation of Microorganism

  实验二十八  微生物抗药性变异

  EXPERIMENT 28  Drug Resistant Variation

  实验二十九  组氨酸营养缺陷型菌株的诱变和筛选

  EXPREIMENT 29  Mutagenesis and Isolation of his Auxotroph

  实验三十  鼠伤寒沙门菌／微粒系统检测化学诱变剂

  EXPERIMENT 30  Ames Test：A Bacterial Test System for Detecting Mutagens andCarcinogens

  实验三十一抗药质粒接合传递实验

  EXPERIMENT 31  Bacterial Conjugation：The Transfer of Antibiotic--Resistant

    Plasmids

  实验三十二  抗药质粒DNA的分离检测和转化

  EXPERIMENT 32  Isolation and Characterization of P1asmid DNA and

    Transformation

  实验三十三  细菌转导

  EXPERIMENT 33  Bacterial Transduction

  实验三十四  枯草杆菌原生质体的制备和再生

  EXPERIMENT 34  Formation and regeneration ofBacillus subtilis protoplast

  实验三十五  利用16s rRNA基因序列进行细菌分类鉴定

  EXPERIMENT 35  Bacterial Identification with 16SrRNA Gene

第六部分  免疫学实验

PART SIX Experiments of Immunology

  实验三十六  抗原与免疫血清的制备

  EXPERIMENT 36  Preparations of Antigen and Antiserum

  实验三十七  吞噬细胞功能的测定

  EXPERIMENT 37  Assay of the Function of Phagocytes

  实验三十八  体液因子的免疫功能测定

  EXPERIMENT 38  Assay of Immune Functions of Humoral Factors

  实验三十九  体液免疫功能检测(1)

  EXPERIMENT 39  Immune Functions by Antibody(1)

  实验四十  体液免疫功能检测(2)

  EXPERIMENT40 Immune Functions of Antibodies(2)

  实验四十一  细胞免疫功能检测(1)——体外实验

  EXPERIMENT 41  Immune Function by Cellular Immunity(1)——in Vitro

  实验四十二  细胞免疫功能检测(2)——细胞因子的检测

  EXPERIMENT 42  Immune Function by Cellular Immunity(2)

    ——Detection of Cytokines Levels

  实验四十三  细胞免疫功能检测(3)——体内实验

  EXPERIMENT 43  Immune Function by Cellular Immunity(3)——in Vivo

第七部分  药学微生物学实验

PART SEVEN  General Mierobial Experiments in Pharmaeeutics

  实验四十四  药物的体外抗菌试验

  EXPERIMENT 44  Determination of Antimicrobial Activityin Vitro

  实验四十五  抗生素效价测定

  Experiment 45  Assay of Antibiotic Potency

  实验四十六  药物的无菌检查法

  EXPERIMENT 46  Sterility Test of Drugs

  实验四十七  药