Part 1  Basic techniques of biochemistry and molecular biology

  Chapter 1  Basic techniques of preparation of biomacromolecules

    Section 1  Salting out

    Section 2  Dialysis and ultrafiltration

  Chapter 2  Spectrophotometry

    Section 1  Basic principles

    Section 2  The basic construction of spectrophotometers

    Section 3  Some home-made spectrophotometers

  Chapter 3  Chromatography

    Section 1  Adsorption chromatography

    Section 2  Partition chromatography

    Section 3  Ion-exchange chromatography

    Section 4  Gel filtration

    Section 5  Affinity chromatography

  Chapter 4  Electrophoretic techniques

    Section 1  Basic principles

    Section 2  Several types of commonly used electrophoresis

  Chapter 5  Centrifugation techniques

    Section 1  Basic principle

    Section 2  General description of centrifugation

  Chapter 6  Genetic engineering and analytieal technology of gene expression

    Section 1  Genetic engineering

    Section 2  Polymerase chain reaction

    Section 3  Nucleic acid hybridization

    Section 4  DNA sequencing technology

Part 2  Experiments of the biochemistry and molecular biology

  Chapter 7  Analysis of purification and physicochemical properties of proteins

    Experiment 1  Colorimetric methods for protein determination

    Experiment 2  Isolation and purification of serum y-globulin

    Experiment 3  Cellulose acetate membrane electrophoresis of serum proteins

    Experiment 4  Determination of relative molecular mass of protein in SDS-PAGE

  Chapter 8  Kinetics of enzyme-catalysed reactions

    Experiment 1 The specificity of enzyme and effect of temperature, pH on the enzyme Activity

    Experiment 2  Isolation of alkaline phosphatase and determination of its specific activity

    Experiment 3  Effects of substrates concentration of enzymatic activity: determination of Km of alkaline phosphatase

    Experiment 4  Inhibition effect of phosphate on alkaline phosphatase

    Experiment 5  Analysis of lactic dehydrogenase isoenzyme

  Chapter 9  Substance metabolism

    Experiment 1  Extraction and identification of liver glycogen

    Experiment 2  Determination of alanine transaminase activity (Reitman-Frankels'method)

    Experiment 3  Enzyme catalyzed transamination of amino acids detected by paper chromatography

  Chapter 10  Basic experiment of molecular biology

    Experiment 1  Preparation of the genomic DNA in the eukaryotic cells

    ExPeriment 2  Determination of the concentration and purity of DNA by spectroscopy

    Experiment 3  Agarose gel electrophoresis of nucleic acids

    Experiment 4  Polymerase chain reaction (PCR)

    Experiment 5  Isolation and purification of the DNA fragments

    Experiment 6  DNA ligation--constructing recombinant plasmids

    Experiment 7  Preparation of E.coli competent cells

    Experiment 8  Transformation of Ca2+ competent ceils

    Experiment 9  The screening of recombinant DNA

    Experiment 10  Minipreparation of plasmid DNA

    Experiment 11  Digestion of plasmid DNA with restriction endonucleases

    Experiment 12  Southern blot

    Experiment 13  Expression of exogenous gene in prokaryotic cells

References

Appendix

  Appendix I  The calculation table of ammonium sulfate saturation

  Appendix II  Common buffer solution preparation

  Appendix III  The relative molecular mass and pI of protein

  Appendix IV  Common data of chromatography

  Appendix V  The conversion between centrifugation speed of centrifugation force