Chapter 1

Basics of Physiological-Pharmacological Experimentation

  Unit 1 Objectives and Requirements of Physiological-Pharmacological Experiment

  Unit 2 Process of Experiment Data and Production of Experiment Reports

  Unit 3 Requirements and Safety Rules in Laboratory

  Unit 4 Operation of Biological Data Acquisition System RM-6240

Chapter 2

General Knowledge of Physiological-Pharmacological Experimentation

  Unit 1 Fundamental Principle of Physiological-Pharmacological Experi- mentation

  Unit 2 Preliminary Tests in Physiological-Pharmacological Experimentation

  Unit 3 Statistical Analysis Most Often Used in Scientific Activities

  Unit 4 Statistic Analysis Using Excel

Chapter 3

Basic Operations on Experimental Animals

  Unit 1 Fundamentals of Animal Experiments

  Unit 2 Operations on ExperimentalAnimals

  Unit 3 Gender Identification and Labeling the Animals

  Unit 4 Narcotics and Animal Narcosis

  Unit 5 Sample Collection and Bleeding

  Unit 6 Drug Administration for Laboratory Animals

Chapter 4

Physiological-Pharmacological Experiments

  Experiment 1 Generation and Conduction of Compound Action Potential

  Experiment 2 Actions of Antiepileptic and Anticonvulsants in Mouse Models

    Experiment 2-1 Effect of Drugs on Electric Convulsive Shock

    Experiment 2-2 Effect of Drugs on Convulsion Induced by Central Stimulants

  Experiment 3 Actions of Analgesics in Mouse Models

    Experiment 3-l Analgesic Effect Assayed by Hot-Plate Model

    Experiment 3-2 Analgesic Effect Assayed Using Chemical-Stimulation Model

  Experiment 4 Influence of Stimulus on the Contraction of Skeletal Muscle

  Experiment 5 Determination of ABO Blood Group

  Experiment 6 Effects of Physical and Chemical Factors on Blood Coagulation

  Experiment 7 Recording of Electrocardiogram in Toads

  Experiment 8 Effects of Chemical Agents on the Activity of the Isolated Toad Heart

  Experiment 9 Regulation of Artery Blood Pressure

  Expenment 10 Measurement of Human Blood Pressure

  Experiment 11 Toxic Effects of Cardiac Glycosides on Heart

  Experiment 12 Effect of Drugs on Isolated Intestinal Smooth Muscles

  Experiment 13 Regulation of Respiratory Movements

  Experiment 14 Experimental Pulmonary Edema in Rabbits

  Experiment 15 Factors and Drugs Influencing Urine Output

  Experiment 16 Drug Actions Influenced by Dosage Forms and Delivery Routes

  Experiment 17 Determination of 50% Effective Dose of Pentobarbital

  Experiment 18 Measurement of Concentration-Time Profile of Metronidazole

  Experiment 19 Calculation of the Pharmacokinetic Parameters

  Experiment 20 Intoxication and Detoxification of Organophosphate Agents

  Experiment 21 Effect of Glucocorticoids on Intraabdominal Capillary Permeability in Mice

  Experiment 22 Effect of Anti-inflammatory Agents on Tumescence in Rat Model

  Experiment 23 In-Vitro Experiment of AntibacterialActions

Chapter 5

Expanded Experiments

  Experiment 1 Extraction of Genomic DNA in Peripheral Blood

  Experiment 2 Extraction of mRNA from Liver Tissues

  Experiment 3 Assessment of Gene Transcription Level by Real-Time PCR

  Experiment 4 Assessment of Protein Expression by Western-Blot

  Experiment 5 Isolation and Culture of Mouse Hepatocytes

Appendix

  Table 1 Physiological parameters of experimental animals

  Table 2 Age, body weight and lifetime of adult experimental animals

  Table 3 Formularies of commonly used saline solution

  Table 4 Bleeding methods for experimental animals

  Table 5 Safe and lethal bleeding volume of experimental animals

  Table 6 Scaling factors for cross-species dose calculation

  Table 7 Application route and dose of commonly used anaesthetics

参考文献