Introduction

　1.1 Mycotoxin research progress

　　1.1.1 Types of mycotoxins

　　1.1.2 The hazards of mycotoxins on livestock

　1.2 The impact of mycotoxins on pigs

　　1.2.1 The impact of aflatoxins on pigs

　　1.2.2 The impact of zearalenone on pigs

　　1.2.3 The impact of DON on pigs

　　1.2.4 The impact of T—2 toxin on pigs

　　1.2.5 The impact of ochratoxin on pigs

　　1.2.6 The impact of furmnisin on pigs

　1.3 Mycotoxin contamination in feed

　　1.3.1 The type of mycotox/n and the effect in feed

　　1.3.2 The limits of mycotoxins in the feed

　　1.3.3 Reasons for mycotoxin contamination in feed

　1.4 Feed mycotoxin detoxification method

　　1.4.1 Physical detoxification

　　1.4.2 Chemical detoxification

　　1.4.3 Enzyme detoxificaiton

　　1.4.4 Biological detoxification

　1.5 Purpose and significance and experiment plan of the research

　　1.5.1 Purose and significance of the research

　　1.5.2 Experirrent plan of the research

2 Material and Method

　2.1 Material

　　2.1.1 SWains

　　2.1.2 The main chemical agent and biologic agent

　　2.1.3 Medium preparation

　　2.1. 4 The main equipment

　　2.1.5 The main experimental animal

　2.2 The main experimental content and methods

　　2.2.1 Screening of pmbiotics fermentation conditions and quality evaluation

　　2.2.2 The inhibitory effect of pmbiotics on the growth of Aspergillus parasiticus and its influencing factors

　　2.2.3 The inhibitlon effects of Anti—mycotoxin plants on Aspergillus

　　2.2.4 Method and technology of probiotics on zyrnolysis anti—mycotoxin

　　2.2.5 Probiotics zyrnolite detection and feeding effects experiment

　　2.2.6 Probiotics zymolite effect on glycolysis of mycotoxin in finisher feed

　　2.2.7 Probiotics zymolite effect on glycolysis of mycotoxin in sow feed

　　2.2.8 Establish addition of pmbiotics zymalite in regular feed and feeding effects

　2.3 Data processing

3 Results and analysis

　3.1 Screening of probiotics fermentation conditions and determination of the

　shelf life

　　3.1.1 Screening of probiotics fermentation conditions

　　3.1.2 The determination of the shelf fife of probiotics

　3.2 Quality evaluation of probiotics

　　3.2.1 Determination of organic acids content in probiotics

　　3.2.2 Determination of amino acids in probiotics

　　3.2.3 Determination of inorganic composition of probiotics

　　3.2.4 Determination of heavy metals content of probiotics

　3.3 The inhibitory effect of probiotics on the growth of Aspergillus parasiticus

　and its influencing factors

　　3.3.1 The effects of probiotics on spores activity of Aspergillus parasiticus

　　3.3.2 Probiotics effects on activity of A.parasiticus spores at different pH

　　3.3.3 Probiotics effects on activity of A.parasiticus spores at different

　density

　　3.3.4 The in,pact of pmbiotics on the toxin production of A.parasiticus

　3.4 Anti-mycotoxin plants inhibition effect on Aspergillus parasiticus growth

　　3.4.1 Screening of anti-mycotoxin plant

　　3.4.2 Anti-mycotoxin plants inhibition on A.parasiticus growth

　　3.4.3 The animal experiment of anti-mycotoxin plants

　3.5 Method and technology of probiotics zymolysis anti-mycotoxin plants

　3.6 Probiotics zymolite detection and feeding effects experiment

　　3.6.1 The traits of probiotics zymolite

　　3.6.2 Probiotics effect on the performance of piglets and its side effect

　3.7 Probiotics zymolite effect on glycolysis of mycotoxin in finisher feed

　　3.7.1 Mycotoxin detection in the basal diet

　　3.7.2 Pig growth status

　　3.7.3 Incidence detection

　　3.7.4 Common disease diagnosis of pigs and effects of Probiotics zymolite

　adding feed on pigs

　3.8 Probiotics zymolite effect on glycolysis of mycotoxin in sow feed

　　3.8.1 The impact of probiotics zymolite on sow reproductive performance

　　3.8.2 Sow antibody detection

　3.9 Establish addition of probiotics zymolite in regular feed and feeding effects

　experiment

　　3.9.1 Detection of mycotoxin in normal basal diet

　　3.9.2 Survey of piglets growth performance

　　3.9.3 Piglets antibody detection

4 Discussion

　4.1 Selection and optimize of probiotic fermentation condition

　　4.1.1 The in,pact of temperature on the fermentation of probiotics

　　4.1.2 The impact of pH on the fermentation of probiotics

　　4.1.3 The impact of inoculation amount on the fermentation of probiotics

　4.2 Mycotoxin contamination of pig feed

　4.3 The impact of mycotoxins on pig immunity

　4.4 Probiotics zymolite degradation of mycotoxins in pig feed

5 Conclusion

6 Innovation

References

APPENDIX Ⅰ Market Hog Vaccination Program

APPENDIX Ⅱ Sow Vaccination Program

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